Severe acute respiratory syndrome coronavirus accessory protein 6 is a virion-associated protein and is released from 6 protein-expressing cells.
Identifieur interne : 003593 ( Main/Exploration ); précédent : 003592; suivant : 003594Severe acute respiratory syndrome coronavirus accessory protein 6 is a virion-associated protein and is released from 6 protein-expressing cells.
Auteurs : Cheng Huang [États-Unis] ; C J Peters ; Shinji MakinoSource :
- Journal of virology [ 0022-538X ] ; 2007.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Protéines virales.
- isolement et purification : Virion, Virus du SRAS.
- métabolisme : Protéines virales, Virion, Virus du SRAS.
- Animaux, Cellules Vero, Centrifugation en gradient de densité, Virion, Virosomes, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Centrifugation, Density Gradient, Chlorocebus aethiops, SARS Virus (chemistry), SARS Virus (isolation & purification), SARS Virus (metabolism), Vero Cells, Viral Proteins (analysis), Viral Proteins (metabolism), Virion (chemistry), Virion (isolation & purification), Virion (metabolism), Virosomes (chemistry).
- MESH :
- chemical , analysis : Viral Proteins.
- chemistry : SARS Virus, Virion, Virosomes.
- isolation & purification : SARS Virus, Virion.
- metabolism : SARS Virus, Viral Proteins, Virion.
- Animals, Centrifugation, Density Gradient, Chlorocebus aethiops, Vero Cells.
Abstract
Analysis of severe acute respiratory syndrome coronavirus (SCoV) by either sucrose gradient equilibrium centrifugation or a virus capture assay using an anti-SCoV S protein antibody demonstrated that the SCoV 6 protein, which is one of the accessory proteins of SCoV, was incorporated into virus particles. Coexpression of the SCoV S, M, E, and 6 proteins was sufficient for incorporation of the 6 protein into virus-like particles. Cells transfected with plasmid expressing the 6 protein released SCoV 6 protein; however, infected cells released SCoV 6 protein only in association with SCoV particles.
DOI: 10.1128/JVI.02307-06
PubMed: 17344286
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Analysis of severe acute respiratory syndrome coronavirus (SCoV) by either sucrose gradient equilibrium centrifugation or a virus capture assay using an anti-SCoV S protein antibody demonstrated that the SCoV 6 protein, which is one of the accessory proteins of SCoV, was incorporated into virus particles. Coexpression of the SCoV S, M, E, and 6 proteins was sufficient for incorporation of the 6 protein into virus-like particles. Cells transfected with plasmid expressing the 6 protein released SCoV 6 protein; however, infected cells released SCoV 6 protein only in association with SCoV particles.</div>
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<name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name>
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<country name="États-Unis"><region name="Texas"><name sortKey="Huang, Cheng" sort="Huang, Cheng" uniqKey="Huang C" first="Cheng" last="Huang">Cheng Huang</name>
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